› 3vzx

X-ray diffraction
1.54Å resolution

Crystal structure of PcrB from bacillus subtilis subap. subtilis str. 168

Released:
DOI: 10.2210/pdb3vzx/pdb
PDB entry 3vzx coloured by chain, front view.
PDB entry 3vzx coloured by chain, side view.
PDB entry 3vzx coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
Insights into TIM-barrel prenyl transferase mechanisms: crystal structures of PcrB from Bacillus subtilis and Staphylococcus aureus.
Ren F, Feng X, Ko TP, Huang CH, Hu Y, Chan HC, Liu YL, Wang K, Chen CC, Pang X, He M, Li Y, Oldfield E, Guo RT
Chembiochem 14 195-9 (2013)
PMID: 23322418

Function and Biology Details

Reaction catalysed: 
All-trans-heptaprenyl diphosphate + sn-glycerol 1-phosphate = diphosphate + sn-3-O-(all-trans-heptaprenyl)glycerol 1-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128597 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heptaprenylglyceryl phosphate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 228 amino acids
Theoretical weight: 25.11 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli
UniProt:
  • Canonical: O34790 (Residues: 1-228; Coverage: 100%)
Gene names: BSU06600, pcrB, yerE
Sequence domains: PcrB family
Structure domains: FMN-linked oxidoreductases

Ligands and Environments

2 bound ligands:
Ligand MG 10 x MG
Ligand CL 5 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P21
Unit cell:
a: 54.116Å b: 55.953Å c: 77.271Å
α: 90° β: 108.46° γ: 90°
R-values:
R R work R free
0.182 0.182 0.225
Expression system: Escherichia coli

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Citations

3 review citations

Biosynthesis of archaeal membrane ether lipids.
Jain et al. (2014)
2 more