3w2l

X-ray diffraction
1.64Å resolution

Structure of Trypanosoma cruzi dihydroorotate dehydrogenase in complex with MII-3-169

Released:
Entry authors: Inaoka DK, Iida M, Tabuchi T, Lee N, Matsuoka S, Shiba T, Sakamoto K, Suzuki S, Balogun EO, Nara T, Aoki T, Inoue M, Honma T, Tanaka A, Harada S, Kita K

Function and Biology Details

Reaction catalysed:
(S)-dihydroorotate + fumarate = orotate + succinate
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-175459 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydroorotate dehydrogenase (fumarate) Chains: A, B
Molecule details ›
Chains: A, B
Length: 313 amino acids
Theoretical weight: 34.07 KDa
Source organism: Trypanosoma cruzi strain CL Brener
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4D3W2 (Residues: 2-314; Coverage: 100%)
Gene names: Tc00.1047053508375.50, pyr4, tcdhod2
Sequence domains: Dihydroorotate dehydrogenase
Structure domains:

Ligands and Environments


Cofactor: Ligand FMN 2 x FMN
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P212121
Unit cell:
a: 67.943Å b: 71.982Å c: 129.273Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.136 0.134 0.17
Expression system: Escherichia coli