› 3wcq

X-ray diffraction
0.97Å resolution

Crystal structure analysis of Cyanidioschyzon melorae ferredoxin D58N mutant

Released:
DOI: 10.2210/pdb3wcq/pdb
PDB entry 3wcq coloured by chain, front view.
PDB entry 3wcq coloured by chain, side view.
PDB entry 3wcq coloured by chain, top view.
Source organism: Cyanidioschyzon merolae
Primary publication:
Increasing the electron-transfer ability of Cyanidioschyzon merolae ferredoxin by a one-point mutation--a high resolution and Fe-SAD phasing crystal structure analysis of the Asp58Asn mutant.
Ueno Y, Matsumoto T, Yamano A, Imai T, Morimoto Y
Biochem Biophys Res Commun 436 736-9 (2013)
PMID: 23792094

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183095 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferredoxin Chain: A
Molecule details ›
Chain: A
Length: 97 amino acids
Theoretical weight: 10.58 KDa
Source organism: Cyanidioschyzon merolae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q85FT5 (Residues: 1-97; Coverage: 100%)
Gene name: petF
Sequence domains: 2Fe-2S iron-sulfur cluster binding domain
Structure domains: Ubiquitin-like (UB roll)

Ligands and Environments

1 bound ligand:
Ligand FES 1 x FES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 34.026Å b: 46.527Å c: 66.139Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.152 0.151 0.17
Expression system: Escherichia coli

Quick links

Citations

3 mentions without citation

Reciprocal carbonyl-carbonyl interactions in small molecules and proteins.
Rahim et al. (2017)
2 more