› 3wg6

X-ray diffraction
2.2Å resolution

Crystal structure of conjugated polyketone reductase C1 from Candida parapsilosis complexed with NADPH

Released:
DOI: 10.2210/pdb3wg6/pdb
PDB entry 3wg6 coloured by chain, front view.
PDB entry 3wg6 coloured by chain, side view.
PDB entry 3wg6 coloured by chain, top view.
Source organism: Candida parapsilosis
Primary publication:
Crystal structure of conjugated polyketone reductase (CPR-C1) from Candida parapsilosis IFO 0708 complexed with NADPH.
Qin HM, Yamamura A, Miyakawa T, Kataoka M, Maruoka S, Ohtsuka J, Nagata K, Shimizu S, Tanokura M
Proteins 81 2059-63 (2013)
PMID: 23852710

Function and Biology Details

Reaction catalysed: 
(R)-pantolactone + NADP(+) = 2-dehydropantolactone + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181178 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADPH-dependent conjugated polyketone reductase C1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 307 amino acids
Theoretical weight: 34.82 KDa
Source organism: Candida parapsilosis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q76L37 (Residues: 1-304; Coverage: 100%)
Gene name: cpr-c1
Sequence domains: Aldo/keto reductase family
Structure domains: NADP-dependent oxidoreductase domain

Ligands and Environments

1 bound ligand:
Ligand NDP 4 x NDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P21
Unit cell:
a: 59.07Å b: 84.93Å c: 136.16Å
α: 90° β: 96.17° γ: 90°
R-values:
R R work R free
0.185 0.182 0.242
Expression system: Escherichia coli

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Citations

2 citation in other articles

Sortase A-mediated crosslinked short-chain dehydrogenases/reductases as novel biocatalysts with improved thermostability and catalytic efficiency.
Li et al. (2017)
1 more

1 mention without citation

Complex fitness landscape shapes variation in a hyperpolymorphic species.
Stolyarova et al. (2022)