› 3ws3

X-ray diffraction
2.34Å resolution

Crystal Structure of H-2D in complex with an insulin derived peptide

Released:
DOI: 10.2210/pdb3ws3/pdb
PDB entry 3ws3 coloured by chain, front view.
PDB entry 3ws3 coloured by chain, side view.
PDB entry 3ws3 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Compensatory mechanisms allow undersized anchor-deficient class I MHC ligands to mediate pathogenic autoreactive T cell responses.
Lamont D, Mukherjee G, Kumar PR, Samanta D, McPhee CG, Kay TW, Almo SC, DiLorenzo TP, Serreze DV
J Immunol 193 2135-46 (2014)
PMID: 25063871

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-134258 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
H-2 class I histocompatibility antigen, D-B alpha chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 273 amino acids
Theoretical weight: 31.8 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01899 (Residues: 26-298; Coverage: 81%)
Gene name: H2-D1
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, D
Molecule details ›
Chains: B, D
Length: 100 amino acids
Theoretical weight: 11.84 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01887 (Residues: 21-119; Coverage: 100%)
Gene name: B2m
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Insulin-1 A chain Chains: E, F
Molecule details ›
Chains: E, F
Length: 9 amino acids
Theoretical weight: 1.1 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: P01325 (Residues: 101-107; Coverage: 8%)
Gene names: Ins-1, Ins1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 87.22Å b: 101.13Å c: 117.54Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.217 0.294
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

6 review citations

The importance of the Non Obese Diabetic (NOD) mouse model in autoimmune diabetes.
Pearson et al. (2016)
5 more

2 mentions without citation

Compensatory mechanisms allow undersized anchor-deficient class I MHC ligands to mediate pathogenic autoreactive T cell responses.
Lamont et al. (2014)
1 more