› 3zci

X-ray diffraction
2.2Å resolution

Crystal structure of Helicobacter pylori T4SS protein CagL in a cubic crystal form with a distorted helical conformation of the RGD-motif

Released:
DOI: 10.2210/pdb3zci/pdb
PDB entry 3zci coloured by chain, front view.
PDB entry 3zci coloured by chain, side view.
PDB entry 3zci coloured by chain, top view.
Source organism: Helicobacter pylori 26695
Primary publication:
A helical RGD motif promoting cell adhesion: crystal structures of the Helicobacter pylori type IV secretion system pilus protein CagL.
Barden S, Lange S, Tegtmeyer N, Conradi J, Sewald N, Backert S, Niemann HH
Structure 21 1931-41 (2013)
PMID: 24076404

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127738 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cag pathogenicity island protein (Cag18) Chain: A
Molecule details ›
Chain: A
Length: 220 amino acids
Theoretical weight: 24.94 KDa
Source organism: Helicobacter pylori 26695
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O25272 (Residues: 21-237; Coverage: 92%)
Gene name: HP_0539
Sequence domains: T4SS protein CagL
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1

Ligands and Environments

4 bound ligands:
Ligand SO4 3 x SO4
Ligand DIO 3 x DIO
Ligand BU9 3 x BU9
Ligand CL 1 x CL
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: F23
Unit cell:
a: 181.002Å b: 181.002Å c: 181.002Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.208 0.245
Expression system: Escherichia coli BL21(DE3)

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Citations

15 review citations

Composition, structure and function of the Helicobacter pylori cag pathogenicity island encoded type IV secretion system.
Backert et al. (2015)
14 more

4 mentions without citation

The Helicobacter pylori Cag Type IV Secretion System.
Cover et al. (2020)
3 more