3zh4

X-ray diffraction
1.8Å resolution

crystal structure of S. pneumoniae Hungary 19A MurA1 in complex with citrate

Released:

Function and Biology Details

Reaction catalysed:
Phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-109306 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetylglucosamine 1-carboxyvinyltransferase Chain: A
Molecule details ›
Chain: A
Length: 419 amino acids
Theoretical weight: 45.1 KDa
Source organism: Streptococcus pneumoniae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: B1IBM3 (Residues: 1-419; Coverage: 100%)
Gene names: SPH_1173, murA, murA1
Sequence domains: EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
Structure domains: Enolpyruvate transferase domain

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21212
Unit cell:
a: 106.584Å b: 70.36Å c: 45.241Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.16 0.204
Expression system: Escherichia coli BL21