› 3zla

X-ray diffraction
3.2Å resolution

Crystal structure of the nucleocapsid protein from Bunyamwera virus bound to RNA

Released:
DOI: 10.2210/pdb3zla/pdb
PDB entry 3zla coloured by chain, front view.
PDB entry 3zla coloured by chain, side view.
PDB entry 3zla coloured by chain, top view.
Source organisms:
Primary publication:
Nucleocapsid protein structures from orthobunyaviruses reveal insight into ribonucleoprotein architecture and RNA polymerization.
Ariza A, Tanner SJ, Walter CT, Dent KC, Shepherd DA, Wu W, Matthews SV, Hiscox JA, Green TJ, Luo M, Elliott RM, Fooks AR, Ashcroft AE, Stonehouse NJ, Ranson NA, Barr JN, Edwards TA
OpenAccess logo Nucleic Acids Res 41 5912-26 (2013)
PMID: 23595147

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-538325 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Nucleoprotein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 235 amino acids
Theoretical weight: 26.84 KDa
Source organism: Bunyamwera virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P16495 (Residues: 1-233; Coverage: 100%)
Gene name: N
Sequence domains: Bunyavirus nucleocapsid (N) protein
Structure domains:
RNA Chains: I, J
Molecule details ›
Chains: I, J
Length: 44 nucleotides
Theoretical weight: 13.43 KDa
Source organism: Escherichia coli BL21(DE3)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: C2
Unit cell:
a: 382.24Å b: 88.724Å c: 88.79Å
α: 90° β: 94.3° γ: 90°
R-values:
R R work R free
0.249 0.247 0.297
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

Orthobunyaviruses: recent genetic and structural insights.
Elliott RM. (2014)
11 more

5 mentions without citation

Multiple origins of viral capsid proteins from cellular ancestors.
Krupovic et al. (2017)
4 more