› 3zrx

X-ray diffraction
1.25Å resolution

The high resolution structure of a dimeric Hamp-Dhp fusion displays strong asymmetry

Released:
DOI: 10.2210/pdb3zrx/pdb
PDB entry 3zrx coloured by chain, front view.
PDB entry 3zrx coloured by chain, side view.
PDB entry 3zrx coloured by chain, top view.
Source organisms:
Primary publication:
Mechanism of regulation of receptor histidine kinases.
Ferris HU, Dunin-Horkawicz S, Hornig N, Hulko M, Martin J, Schultz JE, Zeth K, Lupas AN, Coles M
Structure 20 56-66 (2012)
PMID: 22244755

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128062 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HAMP domain-containing protein; Sensor histidine kinase EnvZ Chains: A, B
Molecule details ›
Chains: A, B
Length: 115 amino acids
Theoretical weight: 13.08 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O28769 (Residues: 278-327; Coverage: 15%)
  • Canonical: P0AEJ4 (Residues: 229-289; Coverage: 14%)
Gene names: AF_1503, JW3367, b3404, envZ, ompB, perA, tpo
Sequence domains:
Structure domains: Helix Hairpins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 36.11Å b: 57.4Å c: 51.62Å
α: 90° β: 107.32° γ: 90°
R-values:
R R work R free
0.153 0.151 0.2
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

Molecular Mechanisms of Two-Component Signal Transduction.
Zschiedrich et al. (2016)
11 more

9 mentions without citation

The accommodation index measures the perturbation associated with insertions and deletions in coiled-coils: Application to understand signaling in histidine kinases.
Schmidt et al. (2017)
8 more