› 3zwo

X-ray diffraction
2Å resolution

Crystal structure of ADP ribosyl cyclase complexed with reaction intermediate

Released:
DOI: 10.2210/pdb3zwo/pdb
PDB entry 3zwo coloured by chain, front view.
PDB entry 3zwo coloured by chain, side view.
PDB entry 3zwo coloured by chain, top view.
Source organism: Aplysia californica
Primary publication:
Structural studies of intermediates along the cyclization pathway of Aplysia ADP-ribosyl cyclase.
Kotaka M, Graeff R, Chen Z, Zhang LH, Lee HC, Hao Q
J Mol Biol 415 514-26 (2012)
PMID: 22138343

Function and Biology Details

Reaction catalysed: 
(1a) NADP(+) = 2'-phospho-cyclic ADP-ribose + nicotinamide
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151371 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 259 amino acids
Theoretical weight: 29.65 KDa
Source organism: Aplysia californica
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P29241 (Residues: 24-282; Coverage: 100%)
Sequence domains: ADP-ribosyl cyclase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand G2Q 6 x G2Q
Ligand NGD 1 x NGD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P1
Unit cell:
a: 60.57Å b: 76.422Å c: 139.71Å
α: 87.8° β: 89.09° γ: 89.32°
R-values:
R R work R free
0.179 0.177 0.228
Expression system: Komagataella pastoris

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Citations

1 review citation

Cyclic ADP-ribose and nicotinic acid adenine dinucleotide phosphate (NAADP) as messengers for calcium mobilization.
Lee HC. (2012)
3 other articles