Function and Biology Details
Reaction catalysed:
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
- not assigned
Biological process:
- not assigned
Cellular component:
- not assigned
Sequence domain:
Structure analysis Details
Assembly composition:
hetero trimer (preferred)
Assembly name:
RISC-loading complex, TARBP2 variant and peptide (preferred)
PDBe Complex ID:
PDB-CPX-165334 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Endoribonuclease Dicer
Molecule details ›
Chains: A, D
Length: 123 amino acids
Theoretical weight: 14.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Dicer, partner-binding domain
Length: 123 amino acids
Theoretical weight: 14.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
Q9UPY3 (Residues: 267-389; Coverage: 6%)
Sequence domains: Dicer, partner-binding domain
RISC-loading complex subunit TARBP2
Molecule details ›
Chains: B, E
Length: 75 amino acids
Theoretical weight: 8.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
Structure domains: Double Stranded RNA Binding Domain
Length: 75 amino acids
Theoretical weight: 8.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: Q15633 (Residues: 289-363; Coverage: 21%)
Structure domains: Double Stranded RNA Binding Domain
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
wwPDB Validation report is not available for this entry.
X-ray source:
ALS BEAMLINE 8.3.1
Spacegroup:
F4132
Expression system: Escherichia coli BL21(DE3)
