› 4a59

X-ray diffraction
2.2Å resolution

Crystal structure of Toxoplasma gondii nucleoside triphosphate diphosphohydrolase 3 (NTPDase3) in complex with AMP

Released:
DOI: 10.2210/pdb4a59/pdb
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Source organism: Toxoplasma gondii
Primary publication:
Structural insight into activation mechanism of Toxoplasma gondii nucleoside triphosphate diphosphohydrolases by disulfide reduction.
Krug U, Zebisch M, Krauss M, Sträter N
J Biol Chem 287 3051-66 (2012)
PMID: 22130673

Function and Biology Details

Reaction catalysed: 
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173290 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nucleoside-triphosphatase 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 611 amino acids
Theoretical weight: 67.66 KDa
Source organism: Toxoplasma gondii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q27893 (Residues: 26-628; Coverage: 100%)
Gene name: NTP3
Sequence domains: GDA1/CD39 (nucleoside phosphatase) family
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand AMP 5 x AMP
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21
Unit cell:
a: 89.17Å b: 166.03Å c: 97.94Å
α: 90° β: 97.15° γ: 90°
R-values:
R R work R free
0.18 0.18 0.228
Expression system: Escherichia coli BL21(DE3)

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Citations

5 review citations

Therapeutic potentials of ecto-nucleoside triphosphate diphosphohydrolase, ecto-nucleotide pyrophosphatase/phosphodiesterase, ecto-5'-nucleotidase, and alkaline phosphatase inhibitors.
al-Rashida et al. (2014)
4 more

2 mentions without citation

Cellular function and molecular structure of ecto-nucleotidases.
Zimmermann et al. (2012)
1 more

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