› 4af2

X-ray diffraction
1.97Å resolution

C61S mutant of thiol peroxidase form E. coli.

Released:
DOI: 10.2210/pdb4af2/pdb
PDB entry 4af2 coloured by chain, front view.
PDB entry 4af2 coloured by chain, side view.
PDB entry 4af2 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The structure of an orthorhombic crystal form of a 'forced reduced' thiol peroxidase reveals lattice formation aided by the presence of the affinity tag.
Beckham KS, Byron O, Roe AJ, Gabrielsen M
Acta Crystallogr Sect F Struct Biol Cryst Commun 68 522-6 (2012)
PMID: 22691780

Function and Biology Details

Reaction catalysed: 
Thioredoxin + ROOH = thioredoxin disulfide + H(2)O + ROH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141668 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiol peroxidase Chain: A
Molecule details ›
Chain: A
Length: 201 amino acids
Theoretical weight: 21.62 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A862 (Residues: 1-168; Coverage: 100%)
Gene names: JW1317, b1324, tpx, yzzJ
Sequence domains: Redoxin
Structure domains: Glutaredoxin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: C2221
Unit cell:
a: 49.37Å b: 71.75Å c: 121.93Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.229 0.283
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Detrimental effect of the 6 His C-terminal tag on YedY enzymatic activity and influence of the TAT signal sequence on YedY synthesis.
Sabaty et al. (2013)

2 mentions without citation

Peroxiredoxins wear many hats: Factors that fashion their peroxide sensing personalities.
Bolduc et al. (2021)
1 more