4am9

X-ray diffraction
2.5Å resolution

CRYSTAL STRUCTURE OF THE YERSINIA ENTEROCOLITICA TYPE III SECRETION CHAPERONE SYCD IN COMPLEX WITH A PEPTIDE OF THE TRANSLOCATOR YOPD

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-131497 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Chaperone SycD Chain: A
Molecule details ›
Chain: A
Length: 148 amino acids
Theoretical weight: 16.49 KDa
Source organism: Yersinia enterocolitica
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O87496 (Residues: 21-163; Coverage: 85%)
Gene name: sycD
Sequence domains: Tetratricopeptide repeat
Structure domains: Tetratricopeptide repeat domain
Type 3 secretion system translocon protein SctB Chain: B
Molecule details ›
Chain: B
Length: 11 amino acids
Theoretical weight: 1.17 KDa
Source organism: Yersinia enterocolitica
Expression system: Not provided
UniProt:
  • Canonical: P37132 (Residues: 56-65; Coverage: 3%)
Gene names: sctB, yopD

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P3121
Unit cell:
a: 106.369Å b: 106.369Å c: 51.998Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.19 0.238
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided