› 4aty

X-ray diffraction
1.85Å resolution

Crystal structure of a Terephthalate 1,2-cis-dihydrodioldehydrogenase from Burkholderia xenovorans LB400

Released:
DOI: 10.2210/pdb4aty/pdb
PDB entry 4aty coloured by chain, front view.
PDB entry 4aty coloured by chain, side view.
PDB entry 4aty coloured by chain, top view.
Source organism: Paraburkholderia xenovorans LB400
Primary publication:
Investigating terephthalate biodegradation: structural characterization of a putative decarboxylating cis-dihydrodiol dehydrogenase.
Bains J, Wulff JE, Boulanger MJ
J Mol Biol 423 284-93 (2012)
PMID: 22889862

Function and Biology Details

Reaction catalysed: 
4-phosphooxy-L-threonine + NAD(+) = 3-amino-2-oxopropyl phosphate + CO(2) + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171878 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-hydroxythreonine-4-phosphate dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 349 amino acids
Theoretical weight: 36.35 KDa
Source organism: Paraburkholderia xenovorans LB400
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13RP4 (Residues: 1-349; Coverage: 100%)
Gene name: Bxe_B2750
Sequence domains: Pyridoxal phosphate biosynthetic protein PdxA
Structure domains: Isopropylmalate Dehydrogenase

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand BME 1 x BME
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: C2221
Unit cell:
a: 44.62Å b: 76.19Å c: 197.48Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.21 0.266
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Microbial synthesis of vanillin from waste poly(ethylene terephthalate).
Sadler et al. (2021)
1 more