› 4awy

X-ray diffraction
1.6Å resolution

Crystal Structure of the Mobile Metallo-beta-Lactamase AIM-1 from Pseudomonas aeruginosa: Insights into Antibiotic Binding and the role of Gln157

Released:
DOI: 10.2210/pdb4awy/pdb
PDB entry 4awy coloured by chain, front view.
PDB entry 4awy coloured by chain, side view.
PDB entry 4awy coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Crystal structure of the mobile metallo-β-lactamase AIM-1 from Pseudomonas aeruginosa: insights into antibiotic binding and the role of Gln157.
Leiros HK, Borra PS, Brandsdal BO, Edvardsen KS, Spencer J, Walsh TR, Samuelsen O
Antimicrob Agents Chemother 56 4341-53 (2012)
PMID: 22664968

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110193 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Metallo-beta-lactamase domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 303 amino acids
Theoretical weight: 31.92 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B5DCA0 (Residues: 1-303; Coverage: 100%)
Gene name: blaAIM-1
Sequence domains: Metallo-beta-lactamase superfamily
Structure domains: Ribonuclease Z/Hydroxyacylglutathione hydrolase-like

Ligands and Environments

3 bound ligands:
Ligand ZN 3 x ZN
Ligand MG 4 x MG
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P6122
Unit cell:
a: 77.831Å b: 77.831Å c: 239.018Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.13 0.129 0.158
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Structural basis for carbapenem-hydrolyzing mechanisms of carbapenemases conferring antibiotic resistance.
Jeon et al. (2015)
6 more

10 mentions without citation

Metallo-β-lactamase structure and function.
Palzkill T. (2013)
9 more