› 4b7j

X-ray diffraction
2.42Å resolution

H1N1 2009 Pandemic Influenza Virus: Resistance of the I223R Neuraminidase Mutant Explained by Kinetic and Structural Analysis

Released:
DOI: 10.2210/pdb4b7j/pdb
PDB entry 4b7j coloured by chain, front view.
PDB entry 4b7j coloured by chain, side view.
PDB entry 4b7j coloured by chain, top view.
Source organism: Influenza A virus
Primary publication:
H1N1 2009 pandemic influenza virus: resistance of the I223R neuraminidase mutant explained by kinetic and structural analysis.
van der Vries E, Collins PJ, Vachieri SG, Xiong X, Liu J, Walker PA, Haire LF, Hay AJ, Schutten M, Osterhaus AD, Martin SR, Boucher CA, Skehel JJ, Gamblin SJ
OpenAccess logo PLoS Pathog 8 e1002914 (2012)
PMID: 23028314

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123733 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neuraminidase Chain: A
Molecule details ›
Chain: A
Length: 469 amino acids
Theoretical weight: 51.7 KDa
Source organism: Influenza A virus
Expression system: Gallus gallus
UniProt:
  • Canonical: F8UU09 (Residues: 1-469; Coverage: 100%)
Gene name: NA
Sequence domains: Neuraminidase
Structure domains: Neuraminidase

Ligands and Environments

3 bound ligands:
Ligand NAG 3 x NAG
Ligand CA 1 x CA
Ligand G39 1 x G39
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P4212
Unit cell:
a: 118.55Å b: 118.55Å c: 67.78Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.189 0.233
Expression system: Gallus gallus

Quick links

Citations

2 review citations

Antiviral natural products and herbal medicines.
Lin et al. (2014)
1 more

5 mentions without citation

H1N1 2009 pandemic influenza virus: resistance of the I223R neuraminidase mutant explained by kinetic and structural analysis.
van der Vries et al. (2012)
4 more