› 4b9f

X-ray diffraction
1.19Å resolution

High resolution structure for family 3a carbohydrate binding module from the cipA scaffolding of clostridium thermocellum

Released:
DOI: 10.2210/pdb4b9f/pdb
PDB entry 4b9f coloured by chain, front view.
PDB entry 4b9f coloured by chain, side view.
PDB entry 4b9f coloured by chain, top view.
Source organism: Acetivibrio thermocellus ATCC 27405
Entry authors: Yaniv O, Shimon LJW, Bayer EA, Lamed R, Frolow F

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170406 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cellulosomal-scaffolding protein A Chains: A, B
Molecule details ›
Chains: A, B
Length: 152 amino acids
Theoretical weight: 16.87 KDa
Source organism: Acetivibrio thermocellus ATCC 27405
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q06851 (Residues: 368-519; Coverage: 8%)
Gene names: Cthe_3077, cipA
Sequence domains: Cellulose binding domain
Structure domains: Endoglucanase-like

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Unit cell:
a: 54.939Å b: 58.868Å c: 87.525Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.152 0.151 0.159
Expression system: Escherichia coli BL21

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