› 4bec

X-ray diffraction
2.84Å resolution

MUTANT (K220A) OF THE HSDR SUBUNIT OF THE ECOR124I RESTRICTION ENZYME IN COMPLEX WITH ATP

Released:
DOI: 10.2210/pdb4bec/pdb
PDB entry 4bec coloured by chain, front view.
PDB entry 4bec coloured by chain, side view.
PDB entry 4bec coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Functional coupling of duplex translocation to DNA cleavage in a type I restriction enzyme.
Csefalvay E, Lapkouski M, Guzanova A, Csefalvay L, Baikova T, Shevelev I, Bialevich V, Shamayeva K, Janscak P, Kuta Smatanova I, Panjikar S, Carey J, Weiserova M, Ettrich R
OpenAccess logo PLoS One 10 e0128700 (2015)
PMID: 26039067

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates, ATP is simultaneously hydrolyzed.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145319 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type I restriction enzyme EcoR124I/EcoR124II endonuclease subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 1038 amino acids
Theoretical weight: 120.22 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P10486 (Residues: 1-1038; Coverage: 100%)
Gene names: hsdR, hsr
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand ATP 2 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P21
Unit cell:
a: 86.329Å b: 124.491Å c: 128.761Å
α: 90° β: 108.31° γ: 90°
R-values:
R R work R free
0.236 0.234 0.28
Expression system: Escherichia coli BL21(DE3)

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Citations

2 citation in other articles

A model for the evolution of prokaryotic DNA restriction-modification systems based upon the structural malleability of Type I restriction-modification enzymes.
Bower et al. (2018)
1 more

4 mentions without citation

Crystal structure of a novel domain of the motor subunit of the Type I restriction enzyme EcoR124 involved in complex assembly and DNA binding.
Grinkevich et al. (2018)
3 more