› 4bl2

X-ray diffraction
2.72Å resolution

Crystal structure of PBP2a clinical mutant E150K from MRSA

Released:
DOI: 10.2210/pdb4bl2/pdb
PDB entry 4bl2 coloured by chain, front view.
PDB entry 4bl2 coloured by chain, side view.
PDB entry 4bl2 coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Disruption of allosteric response as an unprecedented mechanism of resistance to antibiotics.
Fishovitz J, Rojas-Altuve A, Otero LH, Dawley M, Carrasco-López C, Chang M, Hermoso JA, Mobashery S
OpenAccess logo J Am Chem Soc 136 9814-7 (2014)
PMID: 24955778

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101921 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Penicillin binding protein 2 prime Chains: A, B
Molecule details ›
Chains: A, B
Length: 643 amino acids
Theoretical weight: 73.42 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H3JPA5 (Residues: 26-668; Coverage: 96%)
Gene names: SAV0041, mecA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CD 7 x CD
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P212121
Unit cell:
a: 81.437Å b: 101.637Å c: 186.838Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.207 0.287
Expression system: Escherichia coli BL21(DE3)

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Citations

9 review citations

Secondary Bacterial Infections Associated with Influenza Pandemics.
Morris et al. (2017)
8 more

2 mentions without citation

Targeting Antibiotic Resistance.
Chellat et al. (2016)
1 more