› 4bm4

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURE OF MANGANESE PEROXIDASE 4 FROM PLEUROTUS OSTREATUS - CRYSTAL FORM IV

Released:
DOI: 10.2210/pdb4bm4/pdb
PDB entry 4bm4 coloured by chain, front view.
PDB entry 4bm4 coloured by chain, side view.
PDB entry 4bm4 coloured by chain, top view.
Source organism: Pleurotus ostreatus
Primary publication:
Ligninolytic peroxidase genes in the oyster mushroom genome: heterologous expression, molecular structure, catalytic and stability properties, and lignin-degrading ability.
Fernández-Fueyo E, Ruiz-Dueñas FJ, Martínez MJ, Romero A, Hammel KE, Medrano FJ, Martínez AT
OpenAccess logo Biotechnol Biofuels 7 2 (2014)
PMID: 24387130

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197742 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peroxidase Chain: A
Molecule details ›
Chain: A
Length: 338 amino acids
Theoretical weight: 36.35 KDa
Source organism: Pleurotus ostreatus
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: W5IDB6 (Residues: 1-338; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand HEM 1 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: C2
Unit cell:
a: 123.87Å b: 86.5Å c: 41.4Å
α: 90° β: 108.05° γ: 90°
R-values:
R R work R free
0.229 0.225 0.297
Expression system: Escherichia coli K-12

Quick links

Citations

11 review citations

Lignin-degrading enzymes.
Pollegioni et al. (2015)
10 more