› 4bmq

X-ray diffraction
2.2Å resolution

Crystal Structure of Ribonucleotide Reductase apo-NrdF from Bacillus cereus (space group C2)

Released:
DOI: 10.2210/pdb4bmq/pdb
PDB entry 4bmq coloured by chain, front view.
PDB entry 4bmq coloured by chain, side view.
PDB entry 4bmq coloured by chain, top view.
Source organism: Bacillus cereus
Primary publication:
Crystal structure of Bacillus cereus class Ib ribonucleotide reductase di-iron NrdF in complex with NrdI.
Hammerstad M, Hersleth HP, Tomter AB, Røhr AK, Andersson KK
ACS Chem Biol 9 526-37 (2014)
PMID: 24295378

Function and Biology Details

Reaction catalysed: 
2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H(2)O = ribonucleoside diphosphate + thioredoxin
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182524 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonucleoside-diphosphate reductase subunit beta Chains: A, B
Molecule details ›
Chains: A, B
Length: 322 amino acids
Theoretical weight: 37.05 KDa
Source organism: Bacillus cereus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q81G55 (Residues: 1-322; Coverage: 100%)
Gene name: BC_1355
Sequence domains: Ribonucleotide reductase, small chain
Structure domains: Ribonucleotide Reductase, subunit A

Ligands and Environments

1 bound ligand:
Ligand FE2 1 x FE2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: C2
Unit cell:
a: 121.02Å b: 68.2Å c: 87.1Å
α: 90° β: 106.01° γ: 90°
R-values:
R R work R free
0.179 0.176 0.243
Expression system: Escherichia coli

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Citations

3 review citations

Choosing the right metal: case studies of class I ribonucleotide reductases.
Huang et al. (2014)
2 more