› 4bt0

Electron Microscopy
17Å resolution

MuB is an AAAplus ATPase that forms helical filaments to control target selection for DNA transposition

Released:
DOI: 10.2210/pdb4bt0/pdb
PDB entry 4bt0 coloured by chain, front view.
PDB entry 4bt0 coloured by chain, side view.
PDB entry 4bt0 coloured by chain, top view.
Source organism: Muvirus mu
Primary publication:
MuB is an AAA+ ATPase that forms helical filaments to control target selection for DNA transposition.
Mizuno N, Dramićanin M, Mizuuchi M, Adam J, Wang Y, Han YW, Yang W, Steven AC, Mizuuchi K, Ramón-Maiques S
Proc Natl Acad Sci U S A 110 E2441-50 (2013)
PMID: 23776210
Related structures: EMD-2398

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetradecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130472 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Transcriptional regulator (NtrC family) Chain: A
Molecule details ›
Chain: A
Length: 73 amino acids
Theoretical weight: 8.56 KDa
Source organism: Muvirus mu
Expression system: Escherichia coli
UniProt:
  • Canonical: O67198 (Residues: 312-384; Coverage: 17%)
Gene names: aq_1117, ntrC1
Transcriptional regulator (NtrC family) Chain: B
Molecule details ›
Chain: B
Length: 173 amino acids
Theoretical weight: 19.4 KDa
Source organism: Muvirus mu
Expression system: Escherichia coli
UniProt:
  • Canonical: O67198 (Residues: 137-309; Coverage: 39%)
Gene names: aq_1117, ntrC1
Sequence domains: Sigma-54 interaction domain

Ligands and Environments

1 bound ligand:
Ligand ADP 2 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 17Å
Relevant EMDB volumes: EMD-2398
Expression system: Escherichia coli

Quick links

Citations

3 review citations

DNA Transposition at Work.
Hickman et al. (2016)
2 more