Function and Biology Details
Reaction catalysed:
Selective cleavage of Arg-|-Thr and then Arg-|-Ile bonds in prothrombin to form thrombin.
Biochemical function:
Biological process:
Cellular component:
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
Venom prothrombin activator pseutarin-C (preferred)
PDBe Complex ID:
PDB-CPX-176394 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (5 distinct):
Venom prothrombin activator pseutarin-C catalytic subunit
Molecule details ›
Chain: A
Length: 423 amino acids
Theoretical weight: 47.04 KDa
Source organism: Pseudonaja textilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
Length: 423 amino acids
Theoretical weight: 47.04 KDa
Source organism: Pseudonaja textilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
Q56VR3 (Residues: 41-467; Coverage: 95%)
- Vitamin K-dependent carboxylation/gamma-carboxyglutamic (GLA) domain
- EGF-like domain
- Coagulation Factor Xa inhibitory site
- Trypsin
Venom prothrombin activator pseutarin-C non-catalytic subunit
Molecule details ›
Chain: V
Length: 1430 amino acids
Theoretical weight: 162.83 KDa
Source organism: Pseudonaja textilis
Expression system: Cricetulus griseus
UniProt:
Structure domains:
Length: 1430 amino acids
Theoretical weight: 162.83 KDa
Source organism: Pseudonaja textilis
Expression system: Cricetulus griseus
UniProt:
- Canonical: Q7SZN0 (Residues: 31-1460; Coverage: 100%)
Structure domains:
Ligands and Environments
Experiments and Validation Details
X-ray source:
DIAMOND BEAMLINE I04
Spacegroup:
P43212
Expression systems:
- Escherichia coli BL21(DE3)
- Cricetulus griseus
