› 4c4r

X-ray diffraction
1.1Å resolution

Structure of beta-phosphoglucomutase in complex with a phosphonate analogue of beta-glucose-1-phosphate and magnesium trifluoride

Released:
DOI: 10.2210/pdb4c4r/pdb
PDB entry 4c4r coloured by chain, front view.
PDB entry 4c4r coloured by chain, side view.
PDB entry 4c4r coloured by chain, top view.
Source organism: Lactococcus lactis
Primary publication:
α-Fluorophosphonates reveal how a phosphomutase conserves transition state conformation over hexose recognition in its two-step reaction.
Jin Y, Bhattasali D, Pellegrini E, Forget SM, Baxter NJ, Cliff MJ, Bowler MW, Jakeman DL, Blackburn GM, Waltho JP
Proc Natl Acad Sci U S A 111 12384-9 (2014)
PMID: 25104750

Function and Biology Details

Reaction catalysed: 
Beta-D-glucose 1-phosphate = beta-D-glucose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159791 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-phosphoglucomutase Chain: A
Molecule details ›
Chain: A
Length: 221 amino acids
Theoretical weight: 24.24 KDa
Source organism: Lactococcus lactis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P71447 (Residues: 1-221; Coverage: 100%)
Gene names: L0001, LL0429, pgmB
Sequence domains: haloacid dehalogenase-like hydrolase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand YO5 1 x YO5
Ligand MGF 1 x MGF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 38.012Å b: 54.201Å c: 105.407Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.15 0.149 0.173
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Metal Fluorides as Analogues for Studies on Phosphoryl Transfer Enzymes.
Jin et al. (2017)
1 more

4 mentions without citation

α-Fluorophosphonates reveal how a phosphomutase conserves transition state conformation over hexose recognition in its two-step reaction.
Jin et al. (2014)
3 more