› 4c9i

X-ray diffraction
3.1Å resolution

Crystal Structure of the Strawberry Pathogenesis-Related 10 (PR-10) Fra a 1E protein (Form B)

Released:
DOI: 10.2210/pdb4c9i/pdb
PDB entry 4c9i coloured by chain, front view.
PDB entry 4c9i coloured by chain, side view.
PDB entry 4c9i coloured by chain, top view.
Source organism: Fragaria x ananassa
Primary publication:
The strawberry pathogenesis-related 10 (PR-10) Fra a proteins control flavonoid biosynthesis by binding to metabolic intermediates.
Casañal A, Zander U, Muñoz C, Dupeux F, Luque I, Botella MA, Schwab W, Valpuesta V, Marquez JA
OpenAccess logo J Biol Chem 288 35322-32 (2013)
PMID: 24133217

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173224 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Major strawberry allergen Fra a 1-E Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 162 amino acids
Theoretical weight: 17.94 KDa
Source organism: Fragaria x ananassa
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q256S2 (Residues: 2-160; Coverage: 99%)
Gene names: FRAA1E.1, FRAA1E.2
Sequence domains: Pathogenesis-related protein Bet v 1 family
Structure domains: Alpha-D-Glucose-1,6-Bisphosphate; Chain A, domain 4

Ligands and Environments

2 bound ligands:
Ligand GOL 2 x GOL
Ligand KXN 1 x KXN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 81.734Å b: 82.459Å c: 224.664Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.214 0.263
Expression system: Escherichia coli BL21

Quick links

Citations

10 review citations

Ligand Binding of PR-10 Proteins with a Particular Focus on the Bet v 1 Allergen Family.
Aglas et al. (2020)
9 more

2 mentions without citation

The strawberry pathogenesis-related 10 (PR-10) Fra a proteins control flavonoid biosynthesis by binding to metabolic intermediates.
Casañal et al. (2013)
1 more