› 4ce4

Electron Microscopy
4.9Å resolution

39S large subunit of the porcine mitochondrial ribosome

Released:
DOI: 10.2210/pdb4ce4/pdb
PDB entry 4ce4 coloured by chain, front view.
PDB entry 4ce4 coloured by chain, side view.
PDB entry 4ce4 coloured by chain, top view.
Source organism: Sus scrofa domesticus
Primary publication:
Architecture of the large subunit of the mammalian mitochondrial ribosome.
Greber BJ, Boehringer D, Leitner A, Bieri P, Voigts-Hoffmann F, Erzberger JP, Leibundgut M, Aebersold R, Ban N
Nature 505 515-9 (2014)
PMID: 24362565
Related structures: EMD-2490

Function and Biology Details

Reaction catalysed: 
N-substituted aminoacyl-tRNA + H(2)O = N-substituted amino acid + tRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 38-mer (preferred)
PDBe Complex ID:
PDB-CPX-236645 (preferred)
Entry contents:
35 distinct polypeptide molecules
2 distinct RNA molecules
Macromolecules (37 distinct):
39S ribosomal protein L27, mitochondrial Chain: 0
Molecule details ›
Chain: 0
Length: 148 amino acids
Theoretical weight: 15.94 KDa
Source organism: Sus scrofa domesticus
UniProt:
  • Canonical: A0A4X1U0F6 (Residues: 1-148; Coverage: 100%)
Sequence domains: Ribosomal L27 protein
ICT1 Chain: 1
Molecule details ›
Chain: 1
Length: 256 amino acids
Theoretical weight: 30.15 KDa
Source organism: Sus scrofa domesticus
UniProt:
  • Canonical: A0A0R4J8D5 (Residues: 71-97; Coverage: 17%)
Gene name: MRPL18
Large ribosomal subunit protein uL29m Chain: 2
Large ribosomal subunit protein uL30m Chain: 3
Large ribosomal subunit protein bL32m Chain: 5
MRPL33 Chain: 6
Large ribosomal subunit protein bL34m Chain: 7
Large ribosomal subunit protein bL35m Chain: 8
MRPL36 Chain: 9
Large ribosomal subunit protein uL2 RNA-binding domain-containing protein Chain: D
MRPL3 Chain: E
MRPL4 Chain: F
Large ribosomal subunit protein bL9m Chain: I
MRPL13 Chain: N
Large ribosomal subunit protein uL14m Chain: O
MRPL15 Chain: P
Large ribosomal subunit protein uL16m Chain: Q
Large ribosomal subunit protein bL17m Chain: R
ICT1 Chain: S
Large ribosomal subunit protein bL19m Chain: T
MRPL20 Chain: U
Large ribosomal subunit protein bL21m Chain: V
Large ribosomal subunit protein uL22m Chain: W
Large ribosomal subunit protein uL23m Chain: X
Large ribosomal subunit protein uL24m Chain: Y
Large ribosomal subunit protein mL38 Chain: b
TGS domain-containing protein Chain: c
Large ribosomal subunit protein mL44 Chain: h
Large ribosomal subunit protein mL45 Chain: i
ICT1 Chain: l
MRPL52 Chain: o
Large ribosomal subunit protein mL62 Chain: u
UNASSIGNED HELICES Chains: v, w
THIOREDOXIN FOLD Chain: x
UNASSIGNED HELICES Chain: z
16S ribosomal RNA Chain: A
UNASSIGNED RNA Chain: B

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 4.9Å
Relevant EMDB volumes: EMD-2490

Quick links

Citations

47 review citations

Multidimensional proteomics for cell biology.
Larance et al. (2015)
46 more

7 mentions without citation

Principles for Integrative Structural Biology Studies.
Rout et al. (2019)
6 more