› 4cpe

X-ray diffraction
1.06Å resolution

Wild-type streptavidin in complex with love-hate ligand 1 (LH1)

Released:
DOI: 10.2210/pdb4cpe/pdb
PDB entry 4cpe coloured by chain, front view.
PDB entry 4cpe coloured by chain, side view.
PDB entry 4cpe coloured by chain, top view.
Source organism: Streptomyces avidinii
Primary publication:
Love-Hate ligands for high resolution analysis of strain in ultra-stable protein/small molecule interaction.
Fairhead M, Shen D, Chan LK, Lowe ED, Donohoe TJ, Howarth M
Bioorg Med Chem 22 5476-86 (2014)
PMID: 25128469

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chains: A, B
Molecule details ›
Chains: A, B
Length: 127 amino acids
Theoretical weight: 13.28 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22629 (Residues: 37-163; Coverage: 80%)
Sequence domains: Avidin family
Structure domains: Avidin-like

Ligands and Environments

1 bound ligand:
Ligand LUV 2 x LUV
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: I222
Unit cell:
a: 46.59Å b: 93.93Å c: 104.32Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.133 0.132 0.154
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 citation in other articles

Amine Landscaping to Maximize Protein-Dye Fluorescence and Ultrastable Protein-Ligand Interaction.
Jacobsen et al. (2017)
2 more