› 4cph

X-ray diffraction
1.64Å resolution

trans-divalent streptavidin with love-hate ligand 4

Released:
DOI: 10.2210/pdb4cph/pdb
PDB entry 4cph coloured by chain, front view.
PDB entry 4cph coloured by chain, side view.
PDB entry 4cph coloured by chain, top view.
Source organism: Streptomyces avidinii
Primary publication:
Love-Hate ligands for high resolution analysis of strain in ultra-stable protein/small molecule interaction.
Fairhead M, Shen D, Chan LK, Lowe ED, Donohoe TJ, Howarth M
Bioorg Med Chem 22 5476-86 (2014)
PMID: 25128469

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149692 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Streptavidin Chains: A, D
Molecule details ›
Chains: A, D
Length: 127 amino acids
Theoretical weight: 13.25 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22629 (Residues: 37-163; Coverage: 80%)
Sequence domains: Avidin family
Structure domains: Avidin-like
Streptavidin Chains: B, C
Molecule details ›
Chains: B, C
Length: 133 amino acids
Theoretical weight: 14.06 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22629 (Residues: 37-163; Coverage: 80%)
Sequence domains: Avidin family
Structure domains: Avidin-like

Ligands and Environments

1 bound ligand:
Ligand LH4 2 x LH4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: C2
Unit cell:
a: 90.74Å b: 47.49Å c: 104.88Å
α: 90° β: 101.14° γ: 90°
R-values:
R R work R free
0.202 0.201 0.232
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 citation in other articles

Amine Landscaping to Maximize Protein-Dye Fluorescence and Ultrastable Protein-Ligand Interaction.
Jacobsen et al. (2017)
2 more

2 mentions without citation

Conformational dynamics in the Acyl-CoA synthetases, adenylation domains of non-ribosomal peptide synthetases, and firefly luciferase.
Gulick AM. (2009)
1 more