› 4cpi

X-ray diffraction
1.54Å resolution

streptavidin A86D mutant with love-hate ligand 4

Released:
DOI: 10.2210/pdb4cpi/pdb
PDB entry 4cpi coloured by chain, front view.
PDB entry 4cpi coloured by chain, side view.
PDB entry 4cpi coloured by chain, top view.
Source organism: Streptomyces avidinii
Primary publication:
Love-Hate ligands for high resolution analysis of strain in ultra-stable protein/small molecule interaction.
Fairhead M, Shen D, Chan LK, Lowe ED, Donohoe TJ, Howarth M
Bioorg Med Chem 22 5476-86 (2014)
PMID: 25128469

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 127 amino acids
Theoretical weight: 13.33 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22629 (Residues: 37-163; Coverage: 80%)
Sequence domains: Avidin family
Structure domains: Avidin-like

Ligands and Environments

3 bound ligands:
Ligand LH4 4 x LH4
Ligand PEG 4 x PEG
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: C2
Unit cell:
a: 81.26Å b: 81.25Å c: 90.69Å
α: 90° β: 103.84° γ: 90°
R-values:
R R work R free
0.158 0.157 0.182
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 citation in other articles

Amine Landscaping to Maximize Protein-Dye Fluorescence and Ultrastable Protein-Ligand Interaction.
Jacobsen et al. (2017)
2 more

6 mentions without citation

International Union of Basic and Clinical Pharmacology. CII: Pharmacological Modulation of H2S Levels: H2S Donors and H2S Biosynthesis Inhibitors.
Szabo et al. (2017)
5 more