› 4cvu

X-ray diffraction
1.9Å resolution

Structure of Fungal beta-mannosidase from Glycoside Hydrolase Family 2 of Trichoderma harzianum

Released:
DOI: 10.2210/pdb4cvu/pdb
PDB entry 4cvu coloured by chain, front view.
PDB entry 4cvu coloured by chain, side view.
PDB entry 4cvu coloured by chain, top view.
Source organism: Trichoderma harzianum
Primary publication:
Insights into the structure and function of fungal β-mannosidases from glycoside hydrolase family 2 based on multiple crystal structures of the Trichoderma harzianum enzyme.
Nascimento AS, Muniz JR, Aparício R, Golubev AM, Polikarpov I
FEBS J 281 4165-78 (2014)
PMID: 24975648

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100448 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (5 distinct):
Beta-mannosidase A Chain: A
Molecule details ›
Chain: A
Length: 942 amino acids
Theoretical weight: 105.48 KDa
Source organism: Trichoderma harzianum
UniProt:
  • Canonical: A0A075B5H6 (Residues: 1-942; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Ligand NAG 2 x NAG
Ligand BMA 1 x BMA
Ligand MAN 3 x MAN
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
7 bound ligands:
Ligand NAG 7 x NAG
Ligand CD 17 x CD
Ligand NA 6 x NA
Ligand PG4 1 x PG4
Ligand PEG 1 x PEG
Ligand CA 1 x CA
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE D03B-MX1
Spacegroup: P41212
Unit cell:
a: 166.456Å b: 166.456Å c: 121.452Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.234 0.263

Quick links

Citations

3 review citations

A review of the enzymatic hydrolysis of mannans and synergistic interactions between β-mannanase, β-mannosidase and α-galactosidase.
Malgas et al. (2015)
2 more

3 mentions without citation

Insights into the structure and function of fungal β-mannosidases from glycoside hydrolase family 2 based on multiple crystal structures of the Trichoderma harzianum enzyme.
Nascimento et al. (2014)
2 more