4d12

X-ray diffraction
1.4Å resolution

Crystal Structure of Cofactor-free Urate Oxidase Anaerobically Complexed with Uric Acid

Released:

Function and Biology Details

Reaction catalysed:
Urate + O(2) + H(2)O = 5-hydroxyisourate + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169432 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uricase Chain: A
Molecule details ›
Chain: A
Length: 302 amino acids
Theoretical weight: 34.29 KDa
Source organism: Aspergillus flavus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q00511 (Residues: 1-302; Coverage: 100%)
Gene names: Uox, uaZ
Sequence domains: Uricase
Structure domains: Urate Oxidase;

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AGILENT NOVA
Spacegroup: I222
Unit cell:
a: 78.918Å b: 94.87Å c: 104.15Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.119 0.118 0.142
Expression system: Escherichia coli