› 4dgm

X-ray diffraction
1.65Å resolution

Crystal Structure of maize CK2 in complex with the inhibitor apigenin

Released:
DOI: 10.2210/pdb4dgm/pdb
PDB entry 4dgm coloured by chain, front view.
PDB entry 4dgm coloured by chain, side view.
PDB entry 4dgm coloured by chain, top view.
Source organism: Zea mays
Primary publication:
Inhibition of protein kinase CK2 by flavonoids and tyrphostins. A structural insight.
Lolli G, Cozza G, Mazzorana M, Tibaldi E, Cesaro L, Donella-Deana A, Meggio F, Venerando A, Franchin C, Sarno S, Battistutta R, Pinna LA
Biochemistry 51 6097-107 (2012)
PMID: 22794353

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151243 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Casein kinase II subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 326 amino acids
Theoretical weight: 38.61 KDa
Source organism: Zea mays
Expression system: Escherichia coli
UniProt:
  • Canonical: P28523 (Residues: 2-327; Coverage: 98%)
Gene name: ACK2
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand EDO 4 x EDO
Ligand PEG 1 x PEG
Ligand AGI 1 x AGI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: C2
Unit cell:
a: 142.363Å b: 59.875Å c: 45.931Å
α: 90° β: 103.4° γ: 90°
R-values:
R R work R free
0.214 0.212 0.252
Expression system: Escherichia coli

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Citations

21 review citations

Secreted protein kinases.
Tagliabracci et al. (2013)
20 more

3 mentions without citation

Binding site characterization - similarity, promiscuity, and druggability.
Ehrt et al. (2019)
2 more