› 4dkm

X-ray diffraction
1.95Å resolution

Crystal Structure of Amphioxus GFPc1a

Released:
DOI: 10.2210/pdb4dkm/pdb
PDB entry 4dkm coloured by chain, front view.
PDB entry 4dkm coloured by chain, side view.
PDB entry 4dkm coloured by chain, top view.
Source organism: Branchiostoma floridae
Entry authors: Deheyn DD, Bomati EK

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111272 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amphioxus Green Fluorescent Protein, GFPc1a Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 214 amino acids
Theoretical weight: 23.65 KDa
Source organism: Branchiostoma floridae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C3YRA3 (Residues: 3-218; Coverage: 99%)
Gene name: BRAFLDRAFT_75523
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CR2 8 x CR2

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: C2
Unit cell:
a: 158.76Å b: 130.46Å c: 106.33Å
α: 90° β: 128.39° γ: 90°
R-values:
R R work R free
0.293 0.293 0.32
Expression system: Escherichia coli BL21(DE3)

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