› 4dlv

X-ray diffraction
1.57Å resolution

H-Ras Set 2 CaCl2/DTT, ordered off

Released:
DOI: 10.2210/pdb4dlv/pdb
PDB entry 4dlv coloured by chain, front view.
PDB entry 4dlv coloured by chain, side view.
PDB entry 4dlv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Shift in the equilibrium between on and off states of the allosteric switch in Ras-GppNHp affected by small molecules and bulk solvent composition.
Holzapfel G, Buhrman G, Mattos C
Biochemistry 51 6114-26 (2012)
PMID: 22845804

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-133956 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase HRas, N-terminally processed Chain: A
Molecule details ›
Chain: A
Length: 166 amino acids
Theoretical weight: 18.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01112 (Residues: 1-166; Coverage: 88%)
Gene names: HRAS, HRAS1
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

4 bound ligands:
Ligand CA 1 x CA
Ligand MG 3 x MG
Ligand GNP 1 x GNP
Ligand DTT 1 x DTT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: R32
Unit cell:
a: 88.822Å b: 88.822Å c: 134.053Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.188 0.212
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Drugging Ras GTPase: a comprehensive mechanistic and signaling structural view.
Lu et al. (2016)
4 more

2 mentions without citation

A structural model of a Ras-Raf signalosome.
Mysore et al. (2021)
1 more