› 4e2w

X-ray diffraction
1.5Å resolution

X-ray Structure of the H181N mutant of TcaB9, a C-3'-Methyltransferase, in Complex with S-Adenosyl-L-Homocysteine and Sugar Product

Released:
DOI: 10.2210/pdb4e2w/pdb
PDB entry 4e2w coloured by chain, front view.
PDB entry 4e2w coloured by chain, side view.
PDB entry 4e2w coloured by chain, top view.
Source organism: Micromonospora chalcea
Primary publication:
Probing the catalytic mechanism of a C-3'-methyltransferase involved in the biosynthesis of D-tetronitrose.
Bruender NA, Holden HM
Protein Sci 21 876-86 (2012)
PMID: 22495991

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110219 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 416 amino acids
Theoretical weight: 46.07 KDa
Source organism: Micromonospora chalcea
Expression system: Escherichia coli
UniProt:
  • Canonical: B5L6K6 (Residues: 1-414; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ZN 1 x ZN
Ligand EDO 3 x EDO
Ligand SAH 1 x SAH
Ligand JHZ 1 x JHZ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21212
Unit cell:
a: 100.631Å b: 114.891Å c: 37.839Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.189 0.228
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Spirotetronate polyketides as leads in drug discovery.
Lacoske et al. (2015)
3 other articles

1 mention without citation

Probing the catalytic mechanism of a C-3'-methyltransferase involved in the biosynthesis of D-tetronitrose.
Bruender et al. (2012)