› 4e3b

X-ray diffraction
1.5Å resolution

Crystal structure of Tax-Interacting Protein-1 (TIP-1) PDZ domain bound to iCAL36-L (ANSRWPTSIL) peptide

Released:
DOI: 10.2210/pdb4e3b/pdb
PDB entry 4e3b coloured by chain, front view.
PDB entry 4e3b coloured by chain, side view.
PDB entry 4e3b coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Stereochemical determinants of C-terminal specificity in PDZ peptide-binding domains: a novel contribution of the carboxylate-binding loop.
Amacher JF, Cushing PR, Bahl CD, Beck T, Madden DR
J Biol Chem 288 5114-26 (2013)
PMID: 23243314

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127191 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tax1-binding protein 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 102 amino acids
Theoretical weight: 11.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O14907 (Residues: 11-112; Coverage: 82%)
Gene names: TAX1BP3, TIP1
Sequence domains: PDZ domain
Structure domains: Pdz3 Domain
iCAL50 peptide Chains: C, D
Molecule details ›
Chains: C, D
Length: 10 amino acids
Theoretical weight: 1.15 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P1
Unit cell:
a: 27.074Å b: 33.599Å c: 66.882Å
α: 80.11° β: 86.88° γ: 89.21°
R-values:
R R work R free
0.189 0.187 0.216
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

3 review citations

Emerging Themes in PDZ Domain Signaling: Structure, Function, and Inhibition.
Liu et al. (2019)
2 more

2 mentions without citation

PDZ Domain Recognition: Insight from Human Tax-Interacting Protein 1 (TIP-1) Interaction with Target Proteins.
Mohanty et al. (2015)
1 more