› 4e3x

X-ray diffraction
1.24Å resolution

Crystal Structure of Mus musculus 1-pyrroline-5-carboxylate dehydrogenase cryoprotected in proline

Released:
DOI: 10.2210/pdb4e3x/pdb
PDB entry 4e3x coloured by chain, front view.
PDB entry 4e3x coloured by chain, side view.
PDB entry 4e3x coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Proline: Mother Nature's cryoprotectant applied to protein crystallography.
Pemberton TA, Still BR, Christensen EM, Singh H, Srivastava D, Tanner JJ
Acta Crystallogr D Biol Crystallogr 68 1010-8 (2012)
PMID: 22868767

Function and Biology Details

Reaction catalysed: 
L-glutamate 5-semialdehyde + NAD(+) + H(2)O = L-glutamate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183972 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 563 amino acids
Theoretical weight: 61.95 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8CHT0 (Residues: 21-562; Coverage: 96%)
Gene name: Aldh4a1
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand PRO 7 x PRO
Ligand 16P 1 x 16P
Ligand PGE 1 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 84.98Å b: 93.878Å c: 132.406Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.155 0.18
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Proline mechanisms of stress survival.
Liang et al. (2013)
3 more

3 mentions without citation

Structural studies of yeast Δ(1)-pyrroline-5-carboxylate dehydrogenase (ALDH4A1): active site flexibility and oligomeric state.
Pemberton et al. (2014)
2 more