› 4eag

X-ray diffraction
2.7Å resolution

Co-crystal structure of an chimeric AMPK core with ATP

Released:
DOI: 10.2210/pdb4eag/pdb
PDB entry 4eag coloured by chain, front view.
PDB entry 4eag coloured by chain, side view.
PDB entry 4eag coloured by chain, top view.
Source organisms:
Primary publication:
AMP-activated protein kinase undergoes nucleotide-dependent conformational changes.
Chen L, Wang J, Zhang YY, Yan SF, Neumann D, Schlattner U, Wang ZX, Wu JW
Nat Struct Mol Biol 19 716-8 (2012)
PMID: 22659875

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-127583 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
non-specific serine/threonine protein kinase Chain: A
Molecule details ›
Chain: A
Length: 130 amino acids
Theoretical weight: 14.9 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: O18645 (Residues: 458-582; Coverage: 22%)
Gene names: AK, AMPK, AMPK alpha, AMPK-alpha, AMPKalpha, AmpKalpha, CG3051, DmAMPK alpha, Dmel\CG3051, Dmel_CG3051, EG:132E8.2, FBgn0023169, Gprk-4, Gprk4, SNF1A, SNF1A-RA, SNF4A-a, ampk, ampkalpha, dAMPK, dAMPKa, dAMPKalpha, snf1A, snf1a
Sequence domains: Adenylate sensor of SNF1-like protein kinase
Structure domains: Kinase associated domain 1, KA1
5'-AMP-activated protein kinase subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 85 amino acids
Theoretical weight: 9.79 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P80386 (Residues: 187-270; Coverage: 31%)
Gene name: Prkab1
Sequence domains: 5'-AMP-activated protein kinase beta subunit, interaction domain
5'-AMP-activated protein kinase subunit gamma-1 Chain: C
Molecule details ›
Chain: C
Length: 330 amino acids
Theoretical weight: 37.43 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P80385 (Residues: 1-330; Coverage: 100%)
Gene name: Prkag1
Sequence domains: CBS domain

Ligands and Environments

2 bound ligands:
Ligand ATP 2 x ATP
Ligand TAM 1 x TAM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2221
Unit cell:
a: 108.668Å b: 151.283Å c: 109.323Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.208 0.252
Expression system: Escherichia coli

Quick links

Citations

30 review citations

AMPK: Mechanisms of Cellular Energy Sensing and Restoration of Metabolic Balance.
Garcia et al. (2017)
29 more

2 mentions without citation

Deconvoluting AMP-activated protein kinase (AMPK) adenine nucleotide binding and sensing.
Gu et al. (2017)
1 more