› 4eiy

X-ray diffraction
1.8Å resolution

Crystal structure of the chimeric protein of A2aAR-BRIL in complex with ZM241385 at 1.8A resolution

Released:
DOI: 10.2210/pdb4eiy/pdb
PDB entry 4eiy coloured by chain, front view.
PDB entry 4eiy coloured by chain, side view.
PDB entry 4eiy coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for allosteric regulation of GPCRs by sodium ions.
Liu W, Chun E, Thompson AA, Chubukov P, Xu F, Katritch V, Han GW, Roth CB, Heitman LH, IJzerman AP, Cherezov V, Stevens RC
Science 337 232-6 (2012)
PMID: 22798613

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142061 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenosine receptor A2a; Soluble cytochrome b562 Chain: A
Molecule details ›
Chain: A
Length: 447 amino acids
Theoretical weight: 49.97 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P29274 (Residues: 2-208, 219-316; Coverage: 74%)
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
Gene names: ADORA2, ADORA2A, cybC
Sequence domains:
Structure domains:

Ligands and Environments

7 bound ligands:
Ligand ZMA 1 x ZMA
Ligand NA 1 x NA
Ligand CLR 3 x CLR
Ligand OLA 16 x OLA
Ligand OLC 6 x OLC
Ligand OLB 1 x OLB
Ligand PEG 2 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: C2221
Unit cell:
a: 39.442Å b: 179.516Å c: 140.307Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.174 0.213
Expression system: Spodoptera frugiperda

Quick links

Citations

113 review citations

Molecular signatures of G-protein-coupled receptors.
Venkatakrishnan et al. (2013)
112 more

206 mentions without citation

Structure-function of the G protein-coupled receptor superfamily.
Katritch et al. (2013)
205 more