› 4ekn

X-ray diffraction
2.5Å resolution

Structure of the catalytic chain of Methanococcus jannaschii Aspartate Transcarbamoylase in a hexagonal crystal form

Released:
DOI: 10.2210/pdb4ekn/pdb
PDB entry 4ekn coloured by chain, front view.
PDB entry 4ekn coloured by chain, side view.
PDB entry 4ekn coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii DSM 2661
Primary publication:
Structure of the catalytic chain of Methanococcus jannaschii aspartate transcarbamoylase in a hexagonal crystal form: insights into the path of carbamoyl phosphate to the active site of the enzyme.
Vitali J, Singh AK, Soares AS, Colaneri MJ
Acta Crystallogr Sect F Struct Biol Cryst Commun 68 527-34 (2012)
PMID: 22691781

Function and Biology Details

Reaction catalysed: 
Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo trimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-176721 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aspartate carbamoyltransferase catalytic subunit Chain: B
Molecule details ›
Chain: B
Length: 306 amino acids
Theoretical weight: 35.21 KDa
Source organism: Methanocaldococcus jannaschii DSM 2661
Expression system: Escherichia coli
UniProt:
  • Canonical: Q58976 (Residues: 1-306; Coverage: 100%)
Gene names: MJ1581, pyrB
Sequence domains:
Structure domains: Aspartate/ornithine carbamoyltransferase

Ligands and Environments

3 bound ligands:
Ligand SO4 4 x SO4
Ligand K 1 x K
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P6322
Unit cell:
a: 96.96Å b: 96.96Å c: 136.439Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.183 0.27
Expression system: Escherichia coli

Quick links

Citations

1 review citation

From Genome to Structure and Back Again: A Family Portrait of the Transcarbamylases.
Shi et al. (2015)

1 mention without citation

Structure of the catalytic chain of Methanococcus jannaschii aspartate transcarbamoylase in a hexagonal crystal form: insights into the path of carbamoyl phosphate to the active site of the enzyme.
Vitali et al. (2012)