4epl

X-ray diffraction
2.01Å resolution

Crystal Structure of Arabidopsis thaliana GH3.11 (JAR1) in Complex with JA-Ile

Released:

Function and Biology Details

Reaction catalysed:
ATP + jasmonate + an L-amino acid = AMP + diphosphate + a jasmonoyl-L-amino acid
Cellular component:
Sequence domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
PDBe Complex ID:
PDB-CPX-193474 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Jasmonoyl--L-amino acid synthetase JAR1 Chain: A
Molecule details ›
Chain: A
Length: 581 amino acids
Theoretical weight: 65.06 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9SKE2 (Residues: 1-575; Coverage: 100%)
Gene names: At2g46370, F11C10.6, FIN219, JAR1
Sequence domains: GH3 auxin-responsive promoter

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C222
Unit cell:
a: 107.039Å b: 159.024Å c: 76.401Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.169 0.21
Expression system: Escherichia coli