› 4erg

X-ray diffraction
2.79Å resolution

Evidence for a Dual Role of an Active Site Histidine in alpha-Amino-beta-Carboxymuconate-epsilon-Semialdehyde Decarboxylase

Released:
DOI: 10.2210/pdb4erg/pdb
PDB entry 4erg coloured by chain, front view.
PDB entry 4erg coloured by chain, side view.
PDB entry 4erg coloured by chain, top view.
Source organism: Pseudomonas fluorescens
Primary publication:
Evidence for a dual role of an active site histidine in α-amino-β-carboxymuconate-ε-semialdehyde decarboxylase.
Huo L, Fielding AJ, Chen Y, Li T, Iwaki H, Hosler JP, Chen L, Hasegawa Y, Que L, Liu A
Biochemistry 51 5811-21 (2012)
PMID: 22746257

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182963 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amidohydrolase-related domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 334 amino acids
Theoretical weight: 37.21 KDa
Source organism: Pseudomonas fluorescens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q83V25 (Residues: 1-334; Coverage: 100%)
Gene name: nbaD
Sequence domains: Amidohydrolase
Structure domains: Metal-dependent hydrolases

Ligands and Environments

1 bound ligand:
Ligand FE 2 x FE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P42212
Unit cell:
a: 90.425Å b: 90.425Å c: 167.532Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.199 0.273
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 citation in other articles

The power of two: arginine 51 and arginine 239* from a neighboring subunit are essential for catalysis in α-amino-β-carboxymuconate-epsilon-semialdehyde decarboxylase.
Huo et al. (2013)
9 more

2 mentions without citation

Evidence for a dual role of an active site histidine in α-amino-β-carboxymuconate-ε-semialdehyde decarboxylase.
Huo et al. (2012)
1 more