› 4es1

X-ray diffraction
1.1Å resolution

Double-stranded Endonuclease Activity in B. halodurans Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated Cas2 Protein

Released:
DOI: 10.2210/pdb4es1/pdb
PDB entry 4es1 coloured by chain, front view.
PDB entry 4es1 coloured by chain, side view.
PDB entry 4es1 coloured by chain, top view.
Source organism: Alkalihalobacillus halodurans C-125
Primary publication:
Double-stranded endonuclease activity in Bacillus halodurans clustered regularly interspaced short palindromic repeats (CRISPR)-associated Cas2 protein.
Nam KH, Ding F, Haitjema C, Huang Q, DeLisa MP, Ke A
J Biol Chem 287 35943-52 (2012)
PMID: 22942283

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-575622 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRISPR-associated endonuclease Cas2 Chain: A
Molecule details ›
Chain: A
Length: 100 amino acids
Theoretical weight: 11.31 KDa
Source organism: Alkalihalobacillus halodurans C-125
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9KFX8 (Residues: 1-96; Coverage: 100%)
Gene names: BH0342, cas2
Sequence domains: CRISPR associated protein Cas2
Structure domains: Alpha-Beta Plaits

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P21212
Unit cell:
a: 65.887Å b: 33.44Å c: 39.093Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.158 0.164
Expression system: Escherichia coli BL21

Quick links

Citations

18 review citations

An updated evolutionary classification of CRISPR-Cas systems.
Makarova et al. (2015)
17 more

8 mentions without citation

Double-stranded endonuclease activity in Bacillus halodurans clustered regularly interspaced short palindromic repeats (CRISPR)-associated Cas2 protein.
Nam et al. (2012)
7 more