› 4ewf

X-ray diffraction
2Å resolution

The crystal structure of beta-lactamase from Sphaerobacter thermophilus DSM 20745

Released:
DOI: 10.2210/pdb4ewf/pdb
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PDB entry 4ewf coloured by chain, front view.
PDB entry 4ewf coloured by chain, side view.
PDB entry 4ewf coloured by chain, top view.
Source organism: Sphaerobacter thermophilus DSM 20745
Entry authors: Wu R, Clancy S, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112278 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase class A catalytic domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 275 amino acids
Theoretical weight: 29.09 KDa
Source organism: Sphaerobacter thermophilus DSM 20745
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: D1C5R0 (Residues: 1-272; Coverage: 100%)
Gene name: Sthe_2032
Sequence domains: Beta-lactamase enzyme family
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

2 bound ligands:
Ligand SO4 5 x SO4
Ligand ACY 1 x ACY
1 modified residue:
Ligand MSE 28 x MSE

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2221
Unit cell:
a: 137.5Å b: 215.551Å c: 83.816Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.181 0.21
Expression system: Escherichia coli BL21

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