› 4f22

X-ray diffraction
2.06Å resolution

Kainate bound to the K660A mutant of the ligand binding domain of GluA3

Released:
DOI: 10.2210/pdb4f22/pdb
PDB entry 4f22 coloured by chain, front view.
PDB entry 4f22 coloured by chain, side view.
PDB entry 4f22 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
The loss of an electrostatic contact unique to AMPA receptor ligand binding domain 2 slows channel activation.
Holley SM, Ahmed AH, Srinivasan J, Murthy SE, Weiland GA, Oswald RE, Nowak LM
Biochemistry 51 4015-27 (2012)
PMID: 22512472

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148616 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate receptor 3 Chain: A
Molecule details ›
Chain: A
Length: 258 amino acids
Theoretical weight: 28.9 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P19492 (Residues: 417-530, 658-799; Coverage: 30%)
  • Best match: P19492-2 (Residues: 417-530, 658-799)
Gene names: GluA3, Glur3, Gria3
Sequence domains:
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

2 bound ligands:
Ligand KAI 1 x KAI
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P2221
Unit cell:
a: 47.657Å b: 49.028Å c: 136.639Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.181 0.244
Expression system: Escherichia coli

Quick links

Citations

6 citation in other articles

Noncompetitive antagonists induce cooperative AMPA receptor channel gating.
Shi et al. (2019)
5 more

5 mentions without citation

De novo transcriptome analysis of Perna viridis highlights tissue-specific patterns for environmental studies.
Leung et al. (2014)
4 more