› 4fat

X-ray diffraction
1.4Å resolution

Ligand-binding domain of GluA2 (flip) ionotropic glutamate receptor in complex with an allosteric modulator

Released:
DOI: 10.2210/pdb4fat/pdb
PDB entry 4fat coloured by chain, front view.
PDB entry 4fat coloured by chain, side view.
PDB entry 4fat coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Functional analysis of a novel positive allosteric modulator of AMPA receptors derived from a structure-based drug design strategy.
Harms JE, Benveniste M, Maclean JK, Partin KM, Jamieson C
Neuropharmacology 64 45-52 (2013)
PMID: 22735771

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148607 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate receptor 2 Chain: A
Molecule details ›
Chain: A
Length: 263 amino acids
Theoretical weight: 29.18 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P19491 (Residues: 413-796; Coverage: 30%)
  • Best match: P19491-2 (Residues: 413-796)
Gene names: GluA2, Glur2, Gria2
Sequence domains:
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

5 bound ligands:
Ligand GLU 1 x GLU
Ligand 496 1 x 496
Ligand SO4 3 x SO4
Ligand GOL 4 x GOL
Ligand LI 1 x LI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21212
Unit cell:
a: 63.866Å b: 88.143Å c: 47.391Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.168 0.209
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Targeting α-amino-3-hydroxyl-5-methyl-4-isoxazole-propionate receptors in epilepsy.
Citraro et al. (2014)
2 more

6 mentions without citation

Diversity of AMPA Receptor Ligands: Chemotypes, Binding Modes, Mechanisms of Action, and Therapeutic Effects.
Golubeva et al. (2022)
5 more