› 4fce

X-ray diffraction
1.96Å resolution

Crystal structure of Yersinia pestis GlmU in complex with alpha-D-glucosamine 1-phosphate (GP1)

Released:
DOI: 10.2210/pdb4fce/pdb
PDB entry 4fce coloured by chain, front view.
PDB entry 4fce coloured by chain, side view.
PDB entry 4fce coloured by chain, top view.
Source organism: Yersinia pestis CO92
Entry authors: Nocek B, Kuhn M, Gu M, Anderson WF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reactions catalysed: 
Acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-glucosamine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187179 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional protein GlmU Chain: A
Molecule details ›
Chain: A
Length: 459 amino acids
Theoretical weight: 49.17 KDa
Source organism: Yersinia pestis CO92
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8Z9S7 (Residues: 1-456; Coverage: 100%)
Gene names: YPO4119, YP_4026, glmU, y4133
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand GP1 1 x GP1
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P6322
Unit cell:
a: 87.571Å b: 87.571Å c: 251.368Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.185 0.216
Expression system: Escherichia coli

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