› 4fcs

X-ray diffraction
1.5Å resolution

The crystal structures of several mutants of pleurotus eryngii versatile peroxidase

Released:
DOI: 10.2210/pdb4fcs/pdb
PDB entry 4fcs coloured by chain, front view.
PDB entry 4fcs coloured by chain, side view.
PDB entry 4fcs coloured by chain, top view.
Source organism: Pleurotus eryngii
Primary publication:
Two oxidation sites for low redox potential substrates: a directed mutagenesis, kinetic, and crystallographic study on Pleurotus eryngii versatile peroxidase.
Morales M, Mate MJ, Romero A, Martínez MJ, Martínez ÁT, Ruiz-Dueñas FJ
J Biol Chem 287 41053-67 (2012)
PMID: 23071108

Function and Biology Details

Reaction catalysed: 
1-(4-hydroxy-3-methoxyphenyl)-2-(2-methoxyphenoxy)propane-1,3-diol + H(2)O(2) = 4-hydroxy-3-methoxybenzaldehyde + 2-methoxyphenol + glycolaldehyde + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131734 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Versatile peroxidase VPL2 Chain: A
Molecule details ›
Chain: A
Length: 315 amino acids
Theoretical weight: 33.15 KDa
Source organism: Pleurotus eryngii
Expression system: Escherichia coli
UniProt:
  • Canonical: O94753 (Residues: 31-345; Coverage: 93%)
Gene name: vpl2
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
3 bound ligands:
Ligand CA 2 x CA
Ligand SO4 1 x SO4
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: I41
Unit cell:
a: 96.271Å b: 96.271Å c: 98.907Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.15 0.176
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Ligninolytic enzymes and its mechanisms for degradation of lignocellulosic waste in environment.
Kumar et al. (2020)
2 more

3 mentions without citation

A Polymerization-Associated Structural Switch in FtsZ That Enables Treadmilling of Model Filaments.
Wagstaff et al. (2017)
2 more