› 4fd2

X-ray diffraction
3Å resolution

Crystal structure of the C-terminal domain of ClpB

Released:
DOI: 10.2210/pdb4fd2/pdb
PDB entry 4fd2 coloured by chain, front view.
PDB entry 4fd2 coloured by chain, side view.
PDB entry 4fd2 coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
Structural basis for intersubunit signaling in a protein disaggregating machine.
Biter AB, Lee S, Sung N, Tsai FT
Proc Natl Acad Sci U S A 109 12515-20 (2012)
PMID: 22802670

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193125 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chaperone protein ClpB Chains: A, B, D
Molecule details ›
Chains: A, B, D
Length: 308 amino acids
Theoretical weight: 34.93 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9RA63 (Residues: 545-852; Coverage: 36%)
Gene names: TTHA1487, clpB
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ADP 3 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P65
Unit cell:
a: 129.775Å b: 129.775Å c: 129.401Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.279 0.278 0.298
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Protein rescue from aggregates by powerful molecular chaperone machines.
Doyle et al. (2013)
8 more

3 mentions without citation

Structural Elements Regulating AAA+ Protein Quality Control Machines.
Chang et al. (2017)
2 more